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KMID : 0985420140360010039
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Laboratory Medicine and Quality Assurance
2014 Volume.36 No. 1 p.39 ~ p.47
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Quantitation of T-lymphocyte Subsets Using the CELL-DYN Sapphire Automated Haematology Analyser
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Kim Ji-Myung
Ryu Seung
Lim Jin-Sook
Kwon Kye-Chul
Koo Sun-Hoe
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Abstract
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Background:Quantitative analysis of T-lymphocyte subsets is used to assess immune competency. Traditionally, T-lymphocyte subset analysis has been performed using flow cytometry, which requires complex instrumentation and relatively skilled manual operation. We evaluated the performance of an automated haematology analyser, the CELL-DYN Sapphire (CD Sapphire; Abbott Laboratories, USA) for T-lymphocyte subset analysis.
Methods:Quantitative analysis of T-lymphocyte subsets is used to assess immune competency. Traditionally, T-lymphocyte subset analysis has been performed using flow cytometry, which requires complex instrumentation and relatively skilled manual operation. We evaluated the performance of an automated haematology analyser, the CELL-DYN Sapphire (CD Sapphire; Abbott Laboratories, USA) for T-lymphocyte subset analysis.
Results:Results of CD Sapphire-based quantitation of CD3+, CD3+CD4+, and CD3+CD8+ cells showed intra-assay CV of less than 5% for precision and displayed linearity in the ranges of 84 to 5364, 41 to 2615, and 44 to 2800 cells/μL, respectively. There was good correlation among the CD3+, CD3+CD4+, and CD3+CD8+ cell counts as well as in the CD4/CD8 ratio (r=0.987, 0.982, 0.982, and 0.980, respectively) using CD Sapphire and flow cytometry. The mean turnaround time for the CD Sapphire (10.0±0.5 minutes) was significantly less than that for flow cytometry (111.8±8.4 minutes, P<0.001).
Conclusion:T cell subset analysis using the CD Sapphire gives excellent performance and consistent results that correlate well with those obtained by flow cytometry. We conclude that this time-efficient method can replace conventional flow cytometric methods used for measuring T cell subsets.
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KEYWORD
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CELL-DYN Sapphire, Flow cytometry, CD4-positive T-lymphocytes, CD8- positive T-lymphocytes, T lymphocyte subsets
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